Coding

Part:BBa_K4199002

Designed by: Yongzhu Li   Group: iGEM22_NWU-CHINA-A   (2022-09-30)


HrtR

A transcription regulator for heme binding.


Usage and Biology

The interaction of heme iron with HrtR is non-covalent, six-coordinated, and involves two histidines, HIS-72 and HIS-149 HrtR specifically binding to the 15-NT palindrome sequence in the hrtRBA promoter region, which is required for hrtRBA inhibition Hrtr-dna binding is abolished by heme addition, which activates the expression of the HRTB-HRTA (HrtBA) transporter in vitro and in vivo. The use of HrtR as an intracellular heme sensor appears to be conserve in many symbiotic bacteria.[1]


Reference

[1]UR D, Cesselin B, Liebl U, Vos MH, Fernandez A, Brun C, Gruss A, Gaudu P. Discovery of intracellular heme-binding protein HrtR, which controls heme efflux by the conserved HrtB-HrtA transporter in Lactococcus lactis. J Biol Chem. 2012 Feb 10;287(7):4752-8. doi: 10.1074/jbc.M111.297531. Epub 2011 Nov 14. PMID: 22084241; PMCID: PMC3281666.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 249
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 249
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 249
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None